Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Caspase-2L antibody [EPR16790] (ab179519)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16790] to Caspase-2L
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Caspase-2L antibody [EPR16790]
See all Caspase-2L primary antibodies
Description

Rabbit monoclonal [EPR16790] to Caspase-2L
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, NIH/3T3 and C6 whole cell lysates. IHC-P: Human testis and lung cancer tissue. ICC/IF: HeLa cells. Flow cytometry: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16790
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-2L antibody [EPR16790] (ab179519)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Caspase-2L with ab179519 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab179519 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)

All lanes : Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution

Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates treated with 1uM staurosporine for 4 hours
Lane 2 : untreated Jurkat whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Observed MW: 48, 30, 14, 12 kDa
Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)

Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Observed MW: 48,30,12 kDa
Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)

Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/10000 dilution + C6 (Rat glial tumor cells) whole cell lysates at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Observed MW: 48,12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (ab179519)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab179519.
Note: nuclear staining on the germ cells of the Human testis was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (ab179519)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab179519.
Note: nuclear staining on the tumor cells of Human lung cancer was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Caspase-2L antibody [EPR16790] (ab179519)

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-2 with ab179519 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-Caspase-2L antibody [EPR16790] (ab179519)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com