Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-2L antibody [EPR16790] (ab179519)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16790] to Caspase-2L
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspase-2L antibody [EPR16790]
    See all Caspase-2L primary antibodies

  • Description

    Rabbit monoclonal [EPR16790] to Caspase-2L

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, NIH/3T3 and C6 whole cell lysates. IHC-P: Human testis and lung cancer tissue. ICC/IF: HeLa cells. Flow cytometry: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-2L antibody [EPR16790] (ab179519)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Caspase-2L with ab179519 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab179519 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    All lanes : Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates treated with 1uM staurosporine for 4 hours
    Lane 2 : untreated Jurkat whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: 48, 30, 14, 12 kDa

  • Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: 48,30,12 kDa

  • Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/10000 dilution + C6 (Rat glial tumor cells) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: 48,12 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (ab179519)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab179519.
    Note: nuclear staining on the germ cells of the Human testis was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (ab179519)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab179519.
    Note: nuclear staining on the tumor cells of Human lung cancer was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Flow Cytometry - Anti-Caspase-2L antibody [EPR16790] (ab179519)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-2 with ab179519 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-Caspase-2L antibody [EPR16790] (ab179519)
    Anti-Caspase-2L antibody [EPR16790] (ab179519)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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