Caspase-2 Inhibitor Assay Kit (ab102490)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Caspase-2 Inhibitor Assay Kit
See all Caspase-2 kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Assay time
1h 30m -
Product overview
Abcam's Caspase 2 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VDVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 2 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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Notes
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-2 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate VDVAD-AFC 1 x 0.5ml DTT 1 x 100µl -
Research areas
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
Tissue specificity
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
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Alternative names
- Casp 2L Pro
- CASP-2
- Casp2
see all
Images
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Caspase 2 Inhibitor Drug Detection Kit (ab102490)
Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using VDVAD-AFC as caspase 2 substrate and following Caspase 2 Inhibitor Drug Detection Kit (ab102490) protocol. Lanes:
1.- Background Control: no Caspase 2, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 2, +z-VAD-FMK
3.- Positive Control: + Caspase 2, no z-VAD-FMK
4.- Caspase 2 + 2.5µM z-VAD-FMK
5.- Caspase 2 + 10µM z-VAD-FMK
6.- Caspase 2 + 40µM z-VAD-FMK