Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
![Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)](https://www.abcam.com/ps/products/247/ab247301/Images/ab247301-407862-anti-caspase-5-antibody-ep876y-bsa-and-azide-free-western-blot-ht-1080-human.jpg "Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP876Y] to Caspase-5 - BSA and Azide free
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free
See all Caspase-5 primary antibodies -
Description
Rabbit monoclonal [EP876Y] to Caspase-5 - BSA and Azide free -
Host species
Rabbit -
Specificity
Based on amino acids homology, this antibody will not recognize either p20 or p10 subunits of Caspase-5.
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Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
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General notes
Ab247301 is the carrier-free version of ab40887. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247301 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP876Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)Anti-Caspase-5 antibody [EP876Y] (ab40887) at 1/1000 dilution + HT1080 (Human fibrosarcoma epithelial cell) prepared in 1% SDS hot lysis methodwhole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using ab40887, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)](https://www.abcam.com/ps/products/247/ab247301/Images/ab247301-3-anti-caspase-5-antibody-ep876y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)This data was developed using ab40887, the same antibody clone in a different buffer formulation.Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1/50 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)](https://www.abcam.com/ps/products/247/ab247301/Images/ab247301-7-anti-caspase-5-antibody-ep876y-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)This data was developed using ab40887, the same antibody clone in a different buffer formulation.
Purified ab40887 at 1/50 dilution (2µg) immunoprecipitating Caspase-5 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat shock (42? 1h) then recovery for 6h whole cell lysate 10µg
Lane 2 (+): ab40887 + HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40887 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 47 kDa -
![Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)](https://www.abcam.com/ps/products/247/ab247301/Images/ab247301-403193-anti-caspase-5-antibody-ep876y-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301) -
![Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)](https://www.abcam.com/ps/products/247/ab247301/Images/ab247301-5-benefits-of-recombinant-antibodies.png)
Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)