Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP876Y] to Caspase-5 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free
    See all Caspase-5 primary antibodies

  • Description

    Rabbit monoclonal [EP876Y] to Caspase-5 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Based on amino acids homology, this antibody will not recognize either p20 or p10 subunits of Caspase-5. 

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.

  • General notes

    Ab247301 is the carrier-free version of ab40887. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247301 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP876Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    Anti-Caspase-5 antibody [EP876Y] (ab40887) at 1/1000 dilution + HT1080 (Human fibrosarcoma epithelial cell) prepared in 1% SDS hot lysis methodwhole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    This data was developed using ab40887, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    This data was developed using ab40887, the same antibody clone in a different buffer formulation.Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1/50 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)

    This data was developed using ab40887, the same antibody clone in a different buffer formulation.
    Purified ab40887 at 1/50 dilution (2µg) immunoprecipitating Caspase-5 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat shock (42? 1h) then recovery for 6h whole cell lysate 10µg
    Lane 2 (+): ab40887 + HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40887 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 47 kDa

  • Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    Anti-Caspase-5 antibody [EP876Y] (ab40887) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    This data was developed using ab40887, the same antibody clone in a different buffer formulation.

    The cell lysates were prepared in 1%SDS Hot lysis method.

  • Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)
    Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (ab247301)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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