Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)
![Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)](https://www.abcam.com/ps/products/251/ab251481/Images/ab251481-403437-anti-c-reactive-protein-antibody-epr20063-bsa-and-azide-free-western-blot.jpg "Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20063] to C Reactive Protein - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free
See all C Reactive Protein primary antibodies -
Description
Rabbit monoclonal [EPR20063] to C Reactive Protein - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
-
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab251481 is the carrier-free version of ab207756. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251481 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR20063 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)Lanes 1-2 & 4 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution
Lane 3 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/5000 dilution
Lane 1 : Human serum
Lane 2 : Rat liver lysate
Lane 3 : Rat serum
Lane 4 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab207756, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1, 2, and 4: 30 seconds; Lane 3: 1 second.
Observed MWt: 25 kDa (Human), 30 kDa (Rat-glycosylated).
The molecular weight observed is consistent with what has been described in the literature (PMID: 7093286).
-
![Western blot - Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)](https://www.abcam.com/ps/products/251/ab251481/Images/ab251481-399215-anti-c-reactive-protein-antibody-epr20063-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution + Mouse serum at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 15 secondsThis data was developed using ab207756, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)](https://www.abcam.com/ps/products/251/ab251481/Images/ab251481-3-benefits-of-recombinant-antibodies.png)
Anti-C Reactive Protein antibody [EPR20063] - BSA and Azide free (ab251481)