Human Lp-PLA2 ELISA Kit (ab235643)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 45 pg/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Lp-PLA2 ELISA Kit
See all PLA2G7 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.5% Inter-assay Sample n Mean SD CV% Serum 3 7.4% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
45 pg/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95 93% - 97% Cell culture media 101 99% - 102% Hep Plasma 96 93% - 97% EDTA Plasma 81 72% - 88% Cit plasma 98 97% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Lp-PLA2 ELISA Kit (ab235643) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lp-PLA2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Lp-PLA2 with 45 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Lp-PLA2 Capture Antibody 1 x 600µl 10X Human Lp-PLA2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Lp-PLA2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. -
Tissue specificity
Plasma. -
Involvement in disease
Platelet-activating factor acetylhydrolase deficiency
Asthma
Atopic hypersensitivity -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family. -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Platelet-activating factor acetylhydrolase precursor
- 1 alkyl 2 acetylglycerophosphocholine esterase
- 1-alkyl-2-acetylglycerophosphocholine esterase
see all -
Database links
- Entrez Gene: 7941 Human
- Entrez Gene: 374569 Human
- Omim: 601690 Human
- SwissProt: Q86U10 Human
- SwissProt: Q13093 Human
- Unigene: 584823 Human
- Unigene: 146239 Human
Images
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Other - Human Lp-PLA2 ELISA Kit (ab235643)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Lp-PLA2 Standard Curve ComparisonStandard Curve comparison between Human Lp-PLA2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Lp-PLA2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Lp-PLA2 in human serum, plasma and cell culture supernatant samples.The concentrations of Lp-PLA2 were measured in duplicates, interpolated from the Lp-PLA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:50, plasma (citrate) 1:50, plasma (EDTA) 1:50, and plasma (heparin) 1:50. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lp-PLA2 concentration was determined to be 175 ng/mL in serum, 102 ng/mL in plasma (citrate), 168 ng/mL in plasma (EDTA) and 118 ng/mL in plasma (heparin).
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lp-PLA2 concentration was determined to be 275 ng/mL with a range of 129 – 436 ng/mL.
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Interpolated concentrations of native Lp-PLA2 in human cell culture supernatant samples.The concentrations of Lp-PLA2 were measured in duplicates, interpolated from the Lp-PLA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: Unstimulated 10%, Stimulated 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lp-PLA2 concentration was determined to be 2.1 ng/mL in unstimulated PBMC cell culture supernatant and 9.3 ng/mL in PHA-M Stimulated cell culture supernatant.
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Sandwich ELISA - Human Lp-PLA2 ELISA Kit (ab235643)To learn more about the advantages of recombinant antibodies see here.
