Anti-c-Kit antibody [EPR24708-25] (ab273119)
![Anti-c-Kit antibody [EPR24708-25] (ab273119)](https://www.abcam.com/ps/products/273/ab273119/Images/ab273119-1-anti-ckit-antibody-epr2470825-immunocytochemistry-p815.jpg "Anti-c-Kit antibody [EPR24708-25] (ab273119)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24708-25] to c-Kit
- Suitable for: Flow Cyt, ICC
- Reacts with: Mouse
Overview
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Product name
Anti-c-Kit antibody [EPR24708-25]
See all c-Kit primary antibodies -
Description
Rabbit monoclonal [EPR24708-25] to c-Kit -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICCmore details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: P815 cells. Flow Cyt: Mouse bone marrow cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24708-25 -
Isotype
IgG
Images
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Immunocytochemistry - Anti-c-Kit antibody (ab273119)
Immunofluorescent analysis of fixed, 0.1% TritonX-100 permeabilized P815 cells labelling c-Kit with ab273119 at 1/50 (10.28 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing membranous staining in P815 cell line. Negative control: NIH/3T3 (PMID: 10523834) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow Cytometry - Anti-c-Kit antibody (ab273119)Flow cytometric analysis of Mouse bone marrow cell cells labelling c-Kit with ab273119 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-Lineage Cocktail (CD3+Gr-1+CD11b+CD45R+Ter-119) conjugated to FITC. Gated on viable cells.
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Flow Cytometry - Anti-c-Kit antibody (ab273119)Flow cytometric analysis of Mouse bone marrow cell cells labelling c-Kit with ab273119 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD34 conjugated to BV421. Gated on viable cells.
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Anti-c-Kit antibody (ab273119)