Anti-TIMP1 antibody - Carboxyterminal end (ab38978)
Key features and details
- Rabbit polyclonal to TIMP1 - Carboxyterminal end
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIMP1 antibody - Carboxyterminal end
See all TIMP1 primary antibodies -
Description
Rabbit polyclonal to TIMP1 - Carboxyterminal end -
Host species
Rabbit -
Specificity
This antibody recognizes both reduced and non reduced TIMP1 protein, but does not cross react with other TIMP family members (TIMP2, TIMP3, TIMP4). -
Tested Applications & Species
See all applications and species dataApplication Species sELISA MouseWB Human
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Immunogen
Synthetic peptide based on the carboxyterminal region of human TIMP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIMP1 antibody - Carboxyterminal end (ab38978)All lanes : Anti-TIMP1 antibody - Carboxyterminal end (ab38978) at 1/1000 dilution
Lane 1 : Human TIMP1
Lane 2 : Cell media from human chondrosarcoma (no treatment)
Lane 3 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size: 23 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
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Sandwich ELISA - Anti-TIMP1 antibody - Carboxyterminal end (ab38978)Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml. -
Western blot - Anti-TIMP1 antibody - Carboxyterminal end (ab38978) Image courtesy of Koji Ueda by AbreviewEach lane corresponds to a distinct lung adenocarcinoma patient sample. The primary antibody was used at 1/3000 dilution. 10µg/ml lysate has been added per lane. A sheep anti-rabbit monoclonal conjugated to HRP was used as the secondary antibody at 1/10,000 dilution.
Detection method used was Western Lightning. 3 minutes. Performed under reducing conditions.
Samples blocked using 5% BSA for 1 hour at 25°C.
Non-specific band at 27kDa is thought to be Ig light chain.
