Anti-BRCA2 antibody (ab123491)
Key features and details
- Rabbit polyclonal to BRCA2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRCA2 antibody
See all BRCA2 primary antibodies -
Description
Rabbit polyclonal to BRCA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 450-500 of Human BRCA2 using the numbering given in entry NP_000050.1.
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Positive control
- Whole cell lysate from 293T, HeLa and Jurkat cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRCA2 antibody (ab123491)All lanes : Anti-BRCA2 antibody (ab123491) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 5 : HeLa whole cell lysate at 50 µg
Lane 6 : HeLa whole cell lysate at 15 µg
Predicted band size: 384 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-BRCA2 antibody (ab123491)Detection of Human BRCA2 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123491 at 6 µg/mg lysate.
Subsequent Western blot detection used ab123491 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Western blot - Anti-BRCA2 antibody (ab123491)All lanes : Anti-BRCA2 antibody (ab123491) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa at 50 µg/ml
Lane 2 : Whole cell lysate from HeLa at 15 µg/ml
Lane 3 : Whole cell lysate from 293T at 50 µg/ml
Lane 4 : Whole cell lysate from Jurkat at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 384 kDa
Exposure time: 10 minutes
