Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸

Order now and get it on Thursday February 25, 2021

Key features and details

Rabbit polyclonal to BRCA2
Suitable for: WB, IP
Reacts with: Human
Isotype: IgG

Product name

Anti-BRCA2 antibody
See all BRCA2 primary antibodies
Description

Rabbit polyclonal to BRCA2
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide, corresponding to a region within amino acids 450-500 of Human BRCA2 using the numbering given in entry NP_000050.1.
Positive control
- Whole cell lysate from 293T, HeLa and Jurkat cells

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate

pH 7 to 8
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-BRCA2 antibody (ab123491)

All lanes : Anti-BRCA2 antibody (ab123491) at 0.1 µg/ml

Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 5 : HeLa whole cell lysate at 50 µg
Lane 6 : HeLa whole cell lysate at 15 µg

Predicted band size: 384 kDa

Exposure time: 30 seconds
Immunoprecipitation - Anti-BRCA2 antibody (ab123491)

Detection of Human BRCA2 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123491 at 6 µg/mg lysate.
Subsequent Western blot detection used ab123491 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-BRCA2 antibody (ab123491)

All lanes : Anti-BRCA2 antibody (ab123491) at 0.1 µg/ml

Lane 1 : Whole cell lysate from HeLa at 50 µg/ml
Lane 2 : Whole cell lysate from HeLa at 15 µg/ml
Lane 3 : Whole cell lysate from 293T at 50 µg/ml
Lane 4 : Whole cell lysate from Jurkat at 50 µg/ml

Developed using the ECL technique.

Predicted band size: 384 kDa

Exposure time: 10 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com