Anti-BRCA2 antibody (ab9143)
Key features and details
- Rabbit polyclonal to BRCA2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRCA2 antibody
See all BRCA2 primary antibodies -
Description
Rabbit polyclonal to BRCA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human BRCA2.
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Positive control
- Purchase matching WB positive control:Recombinant Human BRCA2 protein
- IP: HEK-293T cell lysate. WB: HEK293T, OVCAR-3, Hep-G2, K-562, Jurkat, HeLa, U2OS, and MCF-7 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRCA2 antibody (ab9143)All lanes : Anti-BRCA2 antibody (ab9143) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : OVCAR-3 (human ovary epithelial adenocarcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 8 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-BRCA2 antibody (ab9143)ab9143 immunoprecipitating BRCA2 at 6 µg per reaction.
Lane 1: ab9143 in HEK293T cell lysate.
Lane 2 (control): IgG control
For blotting, ab9143 immunoprecipitated BRAC2 at 0.1 µg/mL. Detected by Chemiluminescence.
