Anti-JAM-C antibody (ab224327)
Key features and details
- Rabbit polyclonal to JAM-C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JAM-C antibody
See all JAM-C primary antibodies -
Description
Rabbit polyclonal to JAM-C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human JAM-C aa 100-250.
Database link: Q9BX67 -
Positive control
- WB: BEWO cell lysate. IHC-P: Human testis tissue. ICC/IF: U-251 MG cells.
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General notes
Previously labelled as Junctional Adhesion Molecule C.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human fallopian tube stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows strong positivity in cilia in glandular cells.
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Anti-JAM-C antibody (ab224327) at 1/100 dilution + BEWO cell lysate
Developed using the ECL technique.
Predicted band size: 35 kDa
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Paraffin-embedded human skeletal muscle stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows no cytoplasmic positivity in myocytes as expected.
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Paraffin-embedded human testis stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows strong membranous positivity in cells in seminiferous ducts.
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Paraffin-embedded human kidney stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in cells in tubules.
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PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for JAM-C (green) using ab224327 at 4 µg/ml in ICC/IF.