Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free
    See all PHD1/prolyl hydroxylase primary antibodies

  • Description

    Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247731 is the carrier-free version of ab108980. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247731 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab108980, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    This data was developed using ab108980, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980 Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    This data was developed using ab108980, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980 Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    Immunocytochemistry - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    This data was developed using ab108980, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab108980
  • Flow Cytometry - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    Flow Cytometry - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    This data was developed using ab108980, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
    Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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