Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23893-40] to CCR3 - BSA and Azide free
  • Suitable for: WB, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free
    See all CCR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23893-40] to CCR3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC, SK-MEL-28, Jurkat and THP-1 whole cell lysate. Human lymph node lysate. ICC: SK-MEL-28 and THP-1 cells.

  • General notes

    ab275256 is the carrier-free version of ab227032. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275256 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR23893-40

  • Isotype

    IgG

Images

  • Western blot - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    Western blot - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    All lanes : Anti-CCR3 antibody [EPR23893-40] (ab227032) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 2 : SK-MEL-28 (human malignant melanoma), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    This data was developed using ab227032, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID:21165210).

    Exposure time: 3 minutes.

  • Immunocytochemistry - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    Immunocytochemistry - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)

    This data was developed using ab227032, the same antibody clone in a different buffer formulation.

    Immunocytochemistry analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 cells labelling CCR3 with ab227032 at 1/100 (5.46 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing cytoplasmic and weak nuclear staining in SK-MEL-28 cells.

    ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    Western blot - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    All lanes : Anti-CCR3 antibody [EPR23893-40] (ab227032) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 2 : SK-MEL-28 (human malignant melanoma), whole cell lysate
    Lane 3 : THP-1 whole cell lysate plus human CCR3 peptide
    Lane 4 : SK-MEL-28 whole cell lysate plus human CCR3 peptide

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 41 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    This data was developed using ab227032, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    Western blot - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    All lanes : Anti-CCR3 antibody [EPR23893-40] (ab227032) at 1/1000 dilution

    Lane 1 : Human lymph node tissue lysate, whole cell lysate
    Lane 2 : Human lymph node tissue lysate, whole cell lysate plus human CCR3 peptide

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 41 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    This data was developed using ab227032, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunocytochemistry - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    Immunocytochemistry - Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)

    This data was developed using ab227032, the same antibody clone in a different buffer formulation.

    Immunocytochemical analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCR3 with ab227032 at 1/50 (10.92 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing cytoplasmic and weak nuclear staining in THP-1 cells, and there is no staining in THP-1 cells after blocking peptide treatment.

    ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)
    Anti-CCR3 antibody [EPR23893-40] - BSA and Azide free (ab275256)

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