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Anti-Bax antibody [2D2] - BSA and Azide free (ab212790)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [2D2] to Bax - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Bax antibody [2D2] - BSA and Azide free
    See all Bax primary antibodies

  • Description

    Mouse monoclonal [2D2] to Bax - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and MCF7 whole cell lysate. IP: HeLa whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab212790 is the carrier-free version of ab270742. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Bax antibody [2D2] - BSA and Azide free (ab212790)
    Western blot - Anti-Bax antibody [2D2] - BSA and Azide free (ab212790)
    All lanes : Anti-Bax antibody [2D2] (ab270742) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

    Predicted band size: 21 kDa


    Exposure time: 37 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation ab270742, PBS, sodium azide, glycerol and BSA.

  • Immunoprecipitation - Anti-Bax antibody [2D2] - BSA and Azide free (ab212790)
    Immunoprecipitation - Anti-Bax antibody [2D2] - BSA and Azide free (ab212790)

    Bax was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (2μg in 0.35mg lysates) with ab270742 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab270742 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab270742 IP in HeLa whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab270742 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 secs.

    This image was produced using the same antibody clone but in a different formulation ab270742, PBS, sodium azide, glycerol and BSA.

  • Anti-Bax antibody [2D2] - BSA and Azide free (ab212790)
    Anti-Bax antibody [2D2] - BSA and Azide free (ab212790)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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