Anti-B4GALT1 antibody (ab121326)
Key features and details
- Rabbit polyclonal to B4GALT1
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-B4GALT1 antibody -
Description
Rabbit polyclonal to B4GALT1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 50-163 of Human B4GALT1 (P15291).
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Positive control
- IHC-P: Human placenta, prostate, and testis tissue sections; WB: HeLa cell lysate; ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-B4GALT1 antibody (ab121326)Immunocytochemistry analysis of A431 (human epidermoid carcinoma) cells labelling B4GALT1 with ab121326 at 2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-B4GALT1 antibody (ab121326)All lanes : Anti-B4GALT1 antibody (ab121326) at 2 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysateLoading control: Anti-HSP90B1
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.