Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)
![Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)](https://www.abcam.com/ps/products/249/ab249079/Images/ab249079-414666-anti-atox1-antibody-epr10352-bsa-and-azide-free-western-blot-hek293-wt-hek293t-atox1-knockout.jpg "Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10352] to ATOX1 - BSA and Azide free
- Suitable for: IP, WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ATOX1 antibody [EPR10352] - BSA and Azide free
See all ATOX1 primary antibodies -
Description
Rabbit monoclonal [EPR10352] to ATOX1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, HepG2 and HeLa cell lysates.
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General notes
Ab249079 is the carrier-free version of ab154179. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249079 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10352 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : ATOX1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 7 kDa
Observed band size: 7 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab154179).
Lanes 1 - 2: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab154179 was shown to react with ATOX1 in HEK-293 wild-type cells in western blot with loss of signal observed in ATOX1 knockout sample. HEK-293 wild-type and ATOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab154179 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)](https://www.abcam.com/ps/products/249/ab249079/Images/ab249079-410357-anti-atox1-antibody-epr10352-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ATOX1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDaThis data was developed using ab154179, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab8245 observed at 36 kDa.
ab154179 Anti-ATOX1 antibody [EPR10352] was shown to specifically react with ATOX1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266651 (knockout cell lysate ab257849) was used. Wild-type and ATOX1 knockout samples were subjected to SDS-PAGE. ab154179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)](https://www.abcam.com/ps/products/249/ab249079/Images/ab249079-402350-anti-atox1-antibody-epr10352-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 7 kDaThis data was developed using ab154179, the same antibody clone in a different buffer formulation.
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![Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)](https://www.abcam.com/ps/products/249/ab249079/Images/ab249079-4-benefits-of-recombinant-antibodies.png)
Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)
