Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22303-124] to ULBP2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free
    See all ULBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22303-124] to ULBP2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    We have preliminary internal testing data to indicate this antibody weakly cross reacts with recombinant human ULBP5 protein in WB. Please contact us for more information.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BxPC-3, MOLT-4, HeLa, HCT116 and U-87 MG whole cell lysates. IP: BxPC-3 whole cell lysate.

  • General notes

    ab275034 is the carrier-free version of ab275023. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275034 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)
    Western blot - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)
    All lanes : Anti-ULBP2 antibody [EPR22303-124] (ab275023) at 1/1000 dilution

    Lane 1 : BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 6 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
    Lane 7 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    This data was developed using ab275023, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds Lane 2-7: 3 minutes.

    Negative control: Raji, Daudi (PMID: 12414645).

  • Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)
    Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)

    This data was developed using ab275023, the same antibody clone in a different buffer formulation.

    ULBP2 was immunoprecipitated from 0.35 mg BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate with ab275023 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275023 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: BxPC-3 whole cell lysate 10 µg

    Lane 2: ab275023 IP in BxPC-3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275023 in BxPC-3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

     

  • Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)
    Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)

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