Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)
![Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)](https://www.abcam.com/ps/products/236/ab236471/Images/ab236471-312600-236471.jpg "Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R6-74-3] to Vitamin D2+Vitamin D3 - BSA and Azide free
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free
See all Vitamin D2+Vitamin D3 primary antibodies -
Description
Rabbit monoclonal [EPPTX-R6-74-3] to Vitamin D2+Vitamin D3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Vitamin D2+Vitamin D3. Vitamin-D-3-BSA.
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General notes
Ab236471 is the carrier-free version of ab229821. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236471 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R6-74-3 -
Isotype
IgG -
Research areas
Images
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Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821).
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 30-35 ng/mL of ab229821 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)](https://www.abcam.com/ps/products/236/ab236471/Images/ab236471-312599-2364712.jpg)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821) used at 8.3 ng/mL.
The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)](https://www.abcam.com/ps/products/236/ab236471/Images/ab236471-3-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)
