Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)
![Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)](https://www.abcam.com/ps/products/229/ab229822/Images/ab229822-1-229822ELISA1.jpg "Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-6-271-12] to Vitamin D2+Vitamin D3
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12]
See all Vitamin D2+Vitamin D3 primary antibodies -
Description
Rabbit monoclonal [EPPTX-6-271-12] to Vitamin D2+Vitamin D3 -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Competitive ELISA: 25-OH-Vitamin-D.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-6-271-12 -
Isotype
IgG -
Research areas
Images
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Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 50-60 ng/mL of ab229822 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)](https://www.abcam.com/ps/products/229/ab229822/Images/ab229822-2-229822ELISA2.jpg)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)ab229822 was used at 11.8 ng/ml.
The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc. -
![Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)](https://www.abcam.com/ps/products/229/ab229822/Images/ab229822-3-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)
