Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)
![Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)](https://www.abcam.com/ps/products/250/ab250312/Images/ab250312-4-anti-pax8-antibody-epr13510-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13510] to PAX8 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-PAX8 antibody [EPR13510] - BSA and Azide free
See all PAX8 primary antibodies -
Description
Rabbit monoclonal [EPR13510] to PAX8 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250312 is the carrier-free version of ab181054. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250312 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13510 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)This data was developed using ab181054, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling PAX8 with purified ab181054 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)](https://www.abcam.com/ps/products/250/ab250312/Images/ab250312-405773-anti-pax8-antibody-epr13510-bsa-and-azide-free-western-blot-human.jpg)
Western blot - Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)All lanes : Anti-PAX8 antibody [EPR13510] (ab181054) at 1/1000 dilution
Lane 1 : SK-OV-3 (Human ovarian cancer epithelial cell) cell lysate
Lane 2 : TT (Human thyroid carcinoma epithelial cell) cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : Human kidney lysate
Lane 5 : Human heart lysate
Lane 6 : Human thyroid lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 30, 50 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsThis data was developed using ab181054, the same antibody clone in a different buffer formulation.
Blocking/Diluting buffer: 5% NFDM/TBST
Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)](https://www.abcam.com/ps/products/250/ab250312/Images/ab250312-2-anti-pax8-antibody-epr13510-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)This data was developed using ab181054, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling PAX8 with unpurified ab181054 at a dilution of 1/500 followed by incubation with a pre-diluted HRP-conjugated secondary antibody and counter-staining with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)](https://www.abcam.com/ps/products/250/ab250312/Images/ab250312-1-anti-pax8-antibody-epr13510-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)This data was developed using ab181054, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PAX8 with unpurified ab181054 at a dilution of 1/500 followed by incubation with a pre-diluted HRP-conjugated secondary antibody and counter-staining with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)](https://www.abcam.com/ps/products/250/ab250312/Images/ab250312-5-benefits-of-recombinant-antibodies.png)
Anti-PAX8 antibody [EPR13510] - BSA and Azide free (ab250312)