Anti-ARIH1 antibody (ab3891)
Key features and details
- Goat polyclonal to ARIH1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARIH1 antibody
See all ARIH1 primary antibodies -
Description
Goat polyclonal to ARIH1 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
HEGYEKDLWEYIED
, corresponding to C terminal amino acids 544-557 of Human ARIH1. -
Positive control
- U937 lysate .
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARIH1 antibody (ab3891) This image is courtesy of an Abreview submitted by T LawsonAll lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilution
Lanes 1-2 : NIH3T3 whole cell lysate at 30 µg
Lane 3 : Partially purified NIH3T3 protein mixture at 10 µg
Secondary
All lanes : Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution
Performed under reducing conditions.
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 15 minutesLanes 1 and 2 - 12% SDS-PAGE.
Lane 3 - 4-15% SDS-PAGE.
Lanes 1 and 3 - developed with colorimetric substrate.
Lane 2 - developed with ECF+.
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Western blot - Anti-ARIH1 antibody (ab3891)ab3891 staining (0.5µ g/ml) of U937 lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.