Anti-ARF GAP 2 antibody [EPR8497] (ab133768)
![Anti-ARF GAP 2 antibody [EPR8497] (ab133768)](https://www.abcam.com/ps/products/133/ab133768/Images/ab133768-147890-anti-arf-gap-2-antibody-epr8497-western-blot.jpg "Anti-ARF GAP 2 antibody [EPR8497] (ab133768)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8497] to ARF GAP 2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ARF GAP 2 antibody [EPR8497]
See all ARF GAP 2 primary antibodies -
Description
Rabbit monoclonal [EPR8497] to ARF GAP 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human ARF GAP 2 aa 300-400. The exact sequence is proprietary.
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Positive control
- Human fetal liver, Human skeletal muscle, HepG2, Human fetal heart and HeLa whole cell lysate (ab150035); Human colon tissue and breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8497 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)All lanes : Anti-ARF GAP 2 antibody [EPR8497] (ab133768) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : HepG2 lysate
Lane 4 : Human fetal heart lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 56 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)](https://www.abcam.com/ps/products/133/ab133768/Images/ab133768-147889-anti-arf-gap-2-antibody-epr8497-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)](https://www.abcam.com/ps/products/133/ab133768/Images/ab133768-147888-anti-arf-gap-2-antibody-epr8497-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)](https://www.abcam.com/ps/products/133/ab133768/Images/ab133768-147887-anti-arf-gap-2-antibody-epr8497.jpg)
OI-RD Scanning - Anti-ARF GAP 2 antibody [EPR8497] (ab133768) -
![Anti-ARF GAP 2 antibody [EPR8497] (ab133768)](https://www.abcam.com/ps/products/133/ab133768/Images/ab133768-5-benefits-of-recombinant-antibodies.png)
Anti-ARF GAP 2 antibody [EPR8497] (ab133768)
