Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (ab252895)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Microsomes, Suspension cells, Tissue Homogenate
- Sensitivity: 0.1 µU
Overview
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Product name
Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells, Microsomes, Tissue Homogenate -
Sensitivity
0.1 µU -
Product overview
In Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (ab252895), FAAH hydrolyzes a non-fluorescent substrate releasing 7-amino-4-methylcoumarin (AMC), a fluorophore, which can be easily measured at Ex/Em= 360/465 nm. The kit provides a specific inhibitor that can be used to compensate for potential non-specfic background in unknown samples. The stable fluorescence signal is positively correlated to FAAH enzymatic activity in samples. The kit offers a rapid, simple, sensitive, reproducible assay and is suitable for detecting FAAH activity as low as 0.1 µU.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AMC Standard (1 mM) 1 x 100µl FAAH Assay Buffer 1 x 25ml FAAH Inhibitor (in DMSO) 1 x 100µl FAAH Positive Control 1 x 40µl FAAH Substrate (in DMSO) 1 x 100µl -
Research areas
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Function
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. -
Tissue specificity
Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. -
Sequence similarities
Belongs to the amidase family. -
Cellular localization
Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network. - Information by UniProt
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Alternative names
- Anandamide amidohydrolase
- Anandamide amidohydrolase 1
- FA2H
see all
Images
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AMC standard curve.
AMC standard curve.
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Kinetic measurement of FAAH activity.Lysates prepared from blank (no sample), rat liver microsome (RLM: 32 µg) and rat liver lysate (RLL: 80 µg) and FAAH Positive Control.
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FAAH specific activity.Activity of two rat liver microsome preparations (RLM1 and RLM2) and rat liver lysate (RLL: 80 µg).