Anti-Aquaporin 2 antibody (ab110496)
Key features and details
- Rabbit polyclonal to Aquaporin 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Aquaporin 2 antibody
See all Aquaporin 2 primary antibodies -
Description
Rabbit polyclonal to Aquaporin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Kidney tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 29 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 29 kDa).Target
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Function
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
Tissue specificity
Expressed in renal collecting tubules. -
Involvement in disease
Diabetes insipidus, nephrogenic, autosomal -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Post-translational
modificationsSer-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. -
Cellular localization
Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 539870 Cow
- Entrez Gene: 359 Human
- Entrez Gene: 11827 Mouse
- Entrez Gene: 25386 Rat
- Omim: 107777 Human
- SwissProt: P79099 Cow
- SwissProt: P79144 Dog
- SwissProt: P41181 Human
see all -
Alternative names
- ADH water channel antibody
- AQP 2 antibody
- AQP CD antibody
see all
Images
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Western blot - Anti-Aquaporin 2 antibody (ab110496)All lanes : Anti-Aquaporin 2 antibody (ab110496) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203) with Immunising peptide at 1 µg/ml
Lane 5 : Kidney (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Kidney (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Aquaporin 2 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab110496 has been referenced in 1 publication.
- McCrimmon A et al. Comprehensive assessment of mitochondrial respiratory function in freshly isolated nephron segments. Am J Physiol Renal Physiol 318:F1237-F1245 (2020). PubMed: 32223308
Images
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Western blot - Anti-Aquaporin 2 antibody (ab110496)All lanes : Anti-Aquaporin 2 antibody (ab110496) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203) with Immunising peptide at 1 µg/ml
Lane 5 : Kidney (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Kidney (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Aquaporin 2 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
