Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21080] to Aquaporin 2
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Aquaporin 2 antibody [EPR21080]
    See all Aquaporin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21080] to Aquaporin 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney lysate; Mouse kidney lysate. IHC-P: Human, mouse and rat kidney tissues. IHC-Fr: Mouse and rat kidney tissues. IP: Aquaporin 2 IP in mouse kidney lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of human kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Western blot - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    All lanes : Anti-Aquaporin 2 antibody [EPR21080] (ab199975) at 1/500 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa



    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds.

    We recommend customers optimize their lysis buffer and sample preparation for WB. This protein might aggregate during heating of samples.

    The molecular mass is consistent with literature (PMID 27405971; PMID 26109089).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of mouse kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of rat kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on mouse kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Nuclear counterstain: DAPI (blue).
    Permeabilization: 0.2% Triton X-100

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on rat kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Nuclear counterstain: DAPI (blue).
    Permeabilization: 0.2% Triton X-100

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Immunoprecipitation - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

    Aquaporin 2 was immunoprecipitated from 0.35 mg mouse kidney lysate with ab199975 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse kidney lysate 10 μg (input).
    Lane 2: ab199975 IP in mouse kidney lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199975 in mouse kidney lysate (-).

    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

    We recommend customers optimize their lysis buffer and sample preparation for IP. This protein might aggregate during heating of samples.

    The molecular mass is consistent with the literature (PMID 27405971; PMID 26109089).

  • Anti-Aquaporin 2 antibody [EPR21080] (ab199975)
    Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

© 2021 Astana Biomed Group LLP. All rights reserved.