Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21080] to Aquaporin 2
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Aquaporin 2 antibody [EPR21080]
See all Aquaporin 2 primary antibodies
Description

Rabbit monoclonal [EPR21080] to Aquaporin 2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal kidney lysate; Mouse kidney lysate. IHC-P: Human, mouse and rat kidney tissues. IHC-Fr: Mouse and rat kidney tissues. IP: Aquaporin 2 IP in mouse kidney lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of human kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

All lanes : Anti-Aquaporin 2 antibody [EPR21080] (ab199975) at 1/500 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Mouse kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 28 kDa

Blocking/dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 10 seconds.

We recommend customers optimize their lysis buffer and sample preparation for WB. This protein might aggregate during heating of samples.

The molecular mass is consistent with literature (PMID 27405971; PMID 26109089).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of mouse kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of rat kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on mouse kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

Nuclear counterstain: DAPI (blue).
Permeabilization: 0.2% Triton X-100

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Immunohistochemical analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on rat kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

Nuclear counterstain: DAPI (blue).
Permeabilization: 0.2% Triton X-100

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.
Immunoprecipitation - Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

Aquaporin 2 was immunoprecipitated from 0.35 mg mouse kidney lysate with ab199975 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Mouse kidney lysate 10 μg (input).
Lane 2: ab199975 IP in mouse kidney lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199975 in mouse kidney lysate (-).

Blocking/dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 10 seconds

We recommend customers optimize their lysis buffer and sample preparation for IP. This protein might aggregate during heating of samples.

The molecular mass is consistent with the literature (PMID 27405971; PMID 26109089).
Anti-Aquaporin 2 antibody [EPR21080] (ab199975)

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