Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)
![Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)](https://www.abcam.com/ps/products/9/ab9400/Images/ab9400-17021-anti-alpha-1-antitrypsin-antibody-g11-western-blot.jpg "Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)")
Key features and details
- Mouse monoclonal [G11] to alpha 1 Antitrypsin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-alpha 1 Antitrypsin antibody [G11]
See all alpha 1 Antitrypsin primary antibodies -
Description
Mouse monoclonal [G11] to alpha 1 Antitrypsin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein.
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General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR3176514-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
G11 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400) This image is courtesy of an Abreview submitted by Mr John AndersenAnti-alpha 1 Antitrypsin antibody [G11] (ab9400) at 1/1000 dilution + Albumin depleted human patient sera, tissue lysate at 50 µg/ml
Secondary
HRP conjugated goat anti-mouse IgG (H&L). at 1/5000 dilution
Observed band size: 52 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)](https://www.abcam.com/ps/products/9/ab9400/Images/ab9400_1.gif)
Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)Western blot using ab9400 at a concentration of 0.5 µg/ml.
Lane 1 - purified alpha 1 antitrypsin
lane 2 - human plasma, 2 µl
lane S - standard proteins
A - SDS-PAGE with Coomasie staining
B - immunoblot with ab9400.Western blot using ab9400 at a concentration of 0.5 µg/ml.
Lane 1 - purified alpha 1 antitrypsin
lane 2 - human plasma, 2 µl
lane S - standard proteins
A - SDS-PAGE with Coomasie staining
B - immunoblot with ab9400.
