Histone H3 Total Acetylation Assay Kit (Fluorometric) (ab131561)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.4 ng
Overview
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Product name
Histone H3 Total Acetylation Assay Kit (Fluorometric)
See all Histone H3 acetylation kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
> 0.4 ng -
Range
0.4 ng - 50 ng -
Assay time
2h 30m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
ab131561 is designed for measuring total histone H3 acetylation in a fast format. In an assay with this kit, the acetyl histone H3 is captured to the strip wells coated with an anti-acetyl histone H3 antibody. The captured acetyl histone H3 can then be detected with a labeled detection antibody followed by a fluorescent development reagent. The ratio of acetyl histone H3 is proportional to the intensity of fluorescence. The absolute amount of acetyl histone H3 can be quantified by comparing to the standard control.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components Identifier 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Assay Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Fluoro Developer 1 x 12µl 1 x 24µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 12µl 1 x 24µl Standard Control (100 µg/mL) 1 x 10µl 1 x 20µl -
Research areas
