Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23324-45] to ADAMTS1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt

Overview

  • Product name

    Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free
    See all ADAMTS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23324-45] to ADAMTS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 4T1 whole cell lysate; Mouse E17 embryo lysate; Mouse kidney lung tissue lysates. Flow cyt: 4T1 cells.

  • General notes

    ab276138 is the carrier-free version of ab276133. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab276138 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)
    Flow Cytometry - Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)

    This data was developed using ab276133, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized 4T1 (mouse mammary gland carcinoma epithelial cell) cells labelling ADAMTS1 with ab276133 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black)  and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)
    Western blot - Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)
    All lanes : Anti-ADAMTS1 antibody [EPR23324-45] (ab276133) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse E17 embryo tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 105 kDa
    Observed band size: 100,120,60-70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab276133, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed are consistent with what have been described in literatures (PMID: 17560840, 23289900, 15184385, 12907688).

    Low expression: mouse brain (PMID: 11108265).

    Exposure time: 15 seconds.

  • Western blot - Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)
    Western blot - Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)
    Anti-ADAMTS1 antibody [EPR23324-45] (ab276133) at 1/1000 dilution + 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell fresh lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 105 kDa
    Observed band size: 100,120 kDa why is the actual band size different from the predicted?



    This data was developed using ab276133, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed are consistent with what have been described in literatures (PMID: 17560840, 23289900, 15184385, 12907688).

    4T1 whole cell lysate is made freshly and used in WB test immediately to minimize protein degradation.

    Exposure time: 92 seconds.

  • Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)
    Anti-ADAMTS1 antibody [EPR23324-45] - BSA and Azide free (ab276138)

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