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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Other ECM Proteins

Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)

Price and availability

606 422 ₸

Availability

Order now and get it on Thursday June 01, 2023

Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR684(2)] to Fibulin-4 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free
    See all Fibulin-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR684(2)] to Fibulin-4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248111 is the carrier-free version of ab125073.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR684(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins

Images

  • Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysate
    Lane 3 : Human fetal lung tissue lysate
    Lane 4 : Human colon cancer tissue lysate
    Lane 5 : Human placenta tissue lysate
    Lane 6 : Mouse placenta tissue lysate
    Lane 7 : Mouse lung tissue lysate
    Lane 8 : NIH/3T3 (mouse embryo) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 49 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab125073, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Human fetal lung tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 49 kDa



    This data was developed using ab125073, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    This data was developed using ab125073, the same antibody clone in a different buffer formulation.ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    This data was developed using ab125073, the same antibody clone in a different buffer formulation.ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
    Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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