Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR684(2)] to Fibulin-4 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free
See all Fibulin-4 primary antibodies -
Description
Rabbit monoclonal [EPR684(2)] to Fibulin-4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248111 is the carrier-free version of ab125073.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR684(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysate
Lane 3 : Human fetal lung tissue lysate
Lane 4 : Human colon cancer tissue lysate
Lane 5 : Human placenta tissue lysate
Lane 6 : Mouse placenta tissue lysate
Lane 7 : Mouse lung tissue lysate
Lane 8 : NIH/3T3 (mouse embryo) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab125073, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDaThis data was developed using ab125073, the same antibody clone in a different buffer formulation.
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This data was developed using ab125073, the same antibody clone in a different buffer formulation.ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab125073, the same antibody clone in a different buffer formulation.ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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