Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5622] to ADAM10 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ADAM10 antibody [EPR5622] - BSA and Azide free
    See all ADAM10 primary antibodies

  • Description

    Rabbit monoclonal [EPR5622] to ADAM10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type HAP1 whole cell lysate; U20S whole cell lysate. IP: LNCaP whole cell lysate.

  • General notes

    Ab242389 is the carrier-free version of ab124695. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242389 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)
    Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty lane
    Lane 3: ADAM10 knockout HAP1 whole cell lysate (40 µg)
    Lane 4: U20S whole cell lysate (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124695 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab124695 was shown to recognize ADAM10 when ADAM10 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE.  Ab124695 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124695).

  • Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)
    Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

    ab124695 (purified) at 1:30 dilution (2ug) immunoprecipitating ADAM-10 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug.
    Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab124695 & LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170952 in HeLa LNCaP (Human prostate carcinoma epithelial cell) whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124695).

  • OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)
    OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124695).

  • Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)
    Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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