Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADAM10 antibody [EPR5622] (ab124695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5622] to ADAM10
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ADAM10 antibody [EPR5622]
    See all ADAM10 primary antibodies

  • Description

    Rabbit monoclonal [EPR5622] to ADAM10

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ADAM10 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat, RAW 264.7, U2OS, LnCaP and MCF-7 cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty lane
    Lane 3: ADAM10 knockout HAP1 whole cell lysate (40 µg)
    Lane 4: U20S whole cell lysate (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124695 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab124695 was shown to recognize ADAM10 when ADAM10 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE.  Ab124695 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)

    ab124695 (purified) at 1:30 dilution (2ug) immunoprecipitating ADAM-10 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug.
    Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab124695 & LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170952 in HeLa LNCaP (Human prostate carcinoma epithelial cell) whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.05 µg/ml (Purified)

    Lane 1 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.09 µg/ml (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)
    OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 84 kDa



    84 kDa band is pro-ADAM10, smaller band at around 65 kDa is mature ADAM10 protein.
  • Anti-ADAM10 antibody [EPR5622] (ab124695)
    Anti-ADAM10 antibody [EPR5622] (ab124695)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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