Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACAT1 antibody [9H10AB4] (ab110290)

Key features and details

  • Mouse monoclonal [9H10AB4] to ACAT1
  • Suitable for: ICC, Flow Cyt, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-ACAT1 antibody [9H10AB4]
    See all ACAT1 primary antibodies

  • Description

    Mouse monoclonal [9H10AB4] to ACAT1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • ICC: Human HDFn cells IHC-P: Human cerebellumIP: HepG2 cells and Human liver mitochondria Flow Cyt: HeLa cells

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    Immunocytochemistry - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    Immunocytochemistry image of ab110290 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (9H10AB4, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • Immunoprecipitation - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    Immunoprecipitation - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    ab110290 immunocaptured from HepG2 cells (lane 1) and Human liver mitochondria (lane 2)

    Predicted molecular weight is 44 kDa.
  • Flow Cytometry - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    Flow Cytometry - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    ab110290, at 1 µg/mL, staining ACAT1 in HeLa (blue) or in an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [9H10AB4] (ab110290)
    ACAT1 immunohistochemistry in human cerebellum visualized with ab110290. ACAT1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of ACAT1 immunoreactivity in the Purkinje cell bodies. The functional significance of this pattern is unknown at present but this antibody offers the opportunity to investigate it in more detail.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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