Human IL-11 ELISA Kit (ab189569)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12 pg/ml
- Range: 43 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-11 ELISA Kit
See all IL-11 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 1.7% Inter-assay Sample n Mean SD CV% Overall 5 10.3% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12 pg/ml -
Range
43 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99.6 94.9% - 104.4% Cell culture media 98.5 93.5% - 104.3% EDTA Plasma 116.9 108.3% - 125.3% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-11 ELISA Kit (ab189569) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-11 protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-11 with 12 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-11 is multifunctional cytokine of IL-6 superfamily. It is secreted by many cell types. IL-11 is best known for its hematopoietic activity. IL-11directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. During embryonic development L-11 signaling is required for normal development of placentation and survival to birth.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-11 Capture Antibody 1 x 600µl 10X Human IL-11 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human IL-11 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipogenesis inhibitory factor
- AGIF
- IL 11
see all -
Database links
- Entrez Gene: 3589 Human
- Omim: 147681 Human
- SwissProt: P20809 Human
- Unigene: 467304 Human
Images
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Other - Human IL-11 ELISA Kit (ab189569)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of IL-11 standard curve for cell culture supernatant samples measurements.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of PMA + IL-1 alpha - treated MRC5 supernatant within the working range of the assay.MRC5 cells were grown in 10F HGDMEM media in the presence of 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 ng/mL IL-1 alpha (ab9616) for 48 hours. IL-11 concentrations were measured in serial dilutions of cell culture supernatants using this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Comparison of IL-11 concentrations in untreated and PMA + IL-1 alpha - treated MRC5 supernatants.MRC5 cells were grown in 10F HGDMEM media in the absence or presence of 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 ng/mL IL-1 alpha (ab9616) for 48 hours. IL-11 concentrations were measured in 8 and 16 X diluted cell culture supernatants using this kit. Interpolated data values adjusted for sample dilution are graphed (mean +/- SD, n=3) in ng of IL-11 per mL of cell culture supernatant.
