Anti-ACAT1 antibody (ab154396)
Key features and details
- Rabbit polyclonal to ACAT1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ACAT1 antibody
See all ACAT1 primary antibodies -
Description
Rabbit polyclonal to ACAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human ACAT1 aa 1-151.
Database link: P24752 -
Positive control
- WB: SW480, HepG2, Jurkat, HEK-293T, A431 and Molt-4 whole cell lysate. Mouse brain whole cell lysate. ICC/IF: HeLa and A431 cells. IHC-P: Human normal kidney tissue.
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ACAT1 knockout SW480 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDaLanes 1 - 4: Merged signal (red and green). Green - ab154396 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab154396 was shown to react with ACAT1 in western blot. The band observed in the knockout lysate lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154396 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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A431 cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.
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Paraffin embedded human normal kidney tissue stained for ACAT1 using ab154396 at 1/1000 dilution in immunohistochemical analysis.
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HeLa cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.
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All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Anti-ACAT1 antibody (ab154396) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE