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Anti-ACAT1 antibody (ab154396)

Anti-ACAT1 antibody (ab154396)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ACAT1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ACAT1 antibody
    See all ACAT1 primary antibodies
  • Description

    Rabbit polyclonal to ACAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human ACAT1 aa 1-151.
    Database link: P24752

  • Positive control

    • WB: SW480, HepG2, Jurkat, HEK-293T, A431 and Molt-4 whole cell lysate. Mouse brain whole cell lysate. ICC/IF: HeLa and A431 cells. IHC-P: Human normal kidney tissue.
  • General notes

    Keep as concentrated solution.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Images

  • Western blot - Anti-ACAT1 antibody (ab154396)
    Western blot - Anti-ACAT1 antibody (ab154396)
    All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution

    Lane 1 : Wild-type SW480 cell lysate
    Lane 2 : ACAT1 knockout SW480 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab154396 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab154396 was shown to react with ACAT1 in western blot. The band observed in the knockout lysate lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154396 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (ab154396)
    Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (ab154396)

    A431 cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody (ab154396)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody (ab154396)

    Paraffin embedded human normal kidney tissue stained for ACAT1 using ab154396 at 1/1000 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (ab154396)
    Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (ab154396)

     HeLa cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF. 

  • Western blot - Anti-ACAT1 antibody (ab154396)
    Western blot - Anti-ACAT1 antibody (ab154396)
    All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution

    Lane 1 : HEK-293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : Molt-4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Western blot - Anti-ACAT1 antibody (ab154396)
    Western blot - Anti-ACAT1 antibody (ab154396)
    Anti-ACAT1 antibody (ab154396) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 45 kDa



    10% SDS PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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