Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free (ab274387)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21995-147] to 4-1BBL - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free
    See all 4-1BBL primary antibodies

  • Description

    Rabbit monoclonal [EPR21995-147] to 4-1BBL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji whole cell lysate. Flow cyt: Raji cells.

  • General notes

    ab274387 is the carrier-free version of ab254385. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab274387 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free (ab274387)
    Western blot - Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free (ab274387)
    All lanes : Anti-4-1BBL antibody [EPR21995-147] (ab254385) at 1/1000 dilution

    Lane 1 : Raji (human Burkitts lymphoma cell line) whole cell lysate at 30 µg
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 60 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 27 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression control: K562 (PMID: 20670353).

    Exposure time:3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254385).

  • Flow Cytometry - Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free (ab274387)
    Flow Cytometry - Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free (ab274387)

    Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling 4-1BBL with ab254385 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254385).

  • Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free (ab274387)
    Anti-4-1BBL antibody [EPR21995-147] - BSA and Azide free (ab274387)

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