Recombinant mouse Wnt3a protein (ab81484)
Key features and details
- Expression system: Cell Culture
- Purity: > 75% SDS-PAGE
- Active: Yes
- Suitable for: CellDiff, SDS-PAGE, Cellular Activation, HPLC
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Product name
Recombinant mouse Wnt3a protein
See all Wnt3a proteins and peptides -
Biological activity
The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.
The expected ED50 for this effect is 0.4-0.6 ng/ml.
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Purity
> 75 % SDS-PAGE.
Greater than 75% by HPLC analyses. -
Expression system
Cell Culture -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
SY PIWWSLAVGP QYSSLSTQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGV KAGIQECQHQ FRGRRWNCTT VSNSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GSAAICGCSS RLQGSPGEGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRTIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNART ERRREKCHCV FHWCCYVSCQ ECTRVYDVHT CK -
Amino acids
19 to 352 -
Additional sequence information
ab81484 is a monomeric glycoprotein containing 334 amino acid residues. This protein is the mature full length (aa19-352), it does not contain the signal peptide from aa 1-18.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (up to 6 months). The lyophilized protein is stable for a few weeks at room temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute to a concentration of 0.1 mg/mL with ddH2O. Store at 4oC for up to 1 week or dilute further in a buffer containing a carrier protein or stabilizer and store working aliquots at -20oC to -80oC for up to 3 months.
Images
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Cell differentiation - Recombinant mouse Wnt3a protein (ab81484) This image is courtesy of Emily Moorefield, Wake Forest Institute for Regenerative Medicine, NC, USAHuman embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.
