Human LYVE ELISA Kit (ab100585)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 8.19 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human LYVE 2 vials LYVE Microplate (12 x 8 wells) 1 unit Recombinant Human LYVE Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
Tissue specificity
Mainly expressed in endothelial cells lining lymphatic vessels. -
Sequence similarities
Contains 1 Link domain. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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Alternative names
- Cell surface retention sequence-binding protein 1
- CRSBP 1
- CRSBP-1
see all -
Database links
- Entrez Gene: 10894 Human
- Omim: 605702 Human
- SwissProt: Q9Y5Y7 Human
- Unigene: 655332 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab100585.
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Typical Standard CurveRepresentative Standard Curve using ab100585.
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ELISA - LYVE Human ELISA Kit (ab100585)ab100585 was used to detect LYVE in Human heparin plasma samples according to the kit protocol. 10 µL sample was used per well.
This image is courtesy of an anonymous Abreview
