Recombinant Mouse sPLA2-IIE protein (His tag) (ab276900)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 97% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Mouse sPLA2-IIE protein (His tag)
See all sPLA2-IIE proteins and peptides -
Purity
> 97 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Mouse -
Sequence
MKPPIALACL CLLVPLAGGN LVQFGVMIER MTGKPALQYN DYGCYCGVGG SHWPVDETDW CCHAHDCCYG RLEKLGCDPK LEKYLFSITR DNIFCAGRTA CQRHTCECDK RAALCFRHNL NTYNRKYAHY PNKLCTGPTP PC -
Predicted molecular weight
16 kDa including tags -
Molecular weight information
The secreted recombinant mouse PLA2G2E comprises 134 amino acids and has a calculated molecular mass of 15.5 kDa. The apparent molecular mass of the recombinant protein is approximately 18 kDa in SDS-PAGE under reducing conditions. -
Amino acids
1 to 142 -
Tags
His tag C-Terminus -
Additional sequence information
Predicted N-terminus: Asn 20.
Specifications
Our Abpromise guarantee covers the use of ab276900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine) -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- 2IIE
- GIIE sPLA2
- Group IIE secretory phospholipase A2
see all -
Function
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids. -
Tissue specificity
Restricted to the brain, heart, lung, and placenta. -
Sequence similarities
Belongs to the phospholipase A2 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant Mouse sPLA2-IIE protein (ab276900)SDS-PAGE analysis of ab276900
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276900 has not yet been referenced specifically in any publications.
Preparation and Storage
- 2IIE
- GIIE sPLA2
- Group IIE secretory phospholipase A2
Images
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SDS-PAGE - Recombinant Mouse sPLA2-IIE protein (ab276900)SDS-PAGE analysis of ab276900