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Neuroscience Neurology process Growth and Development Neurotrophins

Recombinant mouse Midkine protein (ab123474)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

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Description

  • Product name

    Recombinant mouse Midkine protein
    See all Midkine proteins and peptides
  • Biological activity

    Biological Activity: Determined by its ability to chemoattract Human neutrophils using a concentration range of 10-100 ng/ml.
  • Purity

    > 98 % SDS-PAGE.
    Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses.
  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    P12025
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MVAKKKEKVK KGSECSEWTW GPCTPSSKDC GMGFREGTCG AQTQRVHCKV PCNWKKEFGA DCKYKFESWG ACDGSTGTKA RQGTLKKARY NAQCQETIRV TKPCTSKTKS KTKAKKGKGK D
    • Predicted molecular weight

      13 kDa
    • Amino acids

      21 to 140
  • Associated products

    • Related Products

      • Anti-Midkine antibody (ab36038)

    Specifications

    Our Abpromise guarantee covers the use of ab123474 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      HPLC

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    General Info

    • Alternative names

      • Amphiregulin associated protein
      • Amphiregulin-associated protein
      • ARAP
      • FLJ27379
      • Mdk
      • Midgestation and kidney protein
      • Midkine
      • MK
      • MK 1
      • MK_HUMAN
      • MK1
      • NEGF 2
      • NEGF2
      • Neurite growth promoting factor 2
      • Neurite outgrowth promoting protein
      • Neurite outgrowth-promoting factor 2
      • Neurite outgrowth-promoting protein
      • Retinoic acid inducible factor
      see all
    • Function

      Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
    • Sequence similarities

      Belongs to the pleiotrophin family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P21741 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab123474? Please let us know so that we can cite the reference in this datasheet.

    ab123474 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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