Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human Midkine protein (ab49848)

Key features and details

  • Expression system: Escherichia coli
  • Endotoxin level:
  • Suitable for: SDS-PAGE, Functional Studies, WB

Description

  • Product name

    Recombinant Human Midkine protein
    See all Midkine proteins and peptides

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P21741

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

    • Amino acids

      21 to 143

    • Additional sequence information

      Mature protein without signal peptide.

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab49848 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        Functional Studies

        Western blot

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

      • Reconstitution
        For lot specific reconstitution information please contact our Scientific Support Team.

      General Info

      • Alternative names

        • Amphiregulin associated protein
        • Amphiregulin-associated protein
        • ARAP
        • FLJ27379
        • Mdk
        • Midgestation and kidney protein
        • Midkine
        • MK
        • MK 1
        • MK_HUMAN
        • MK1
        • NEGF 2
        • NEGF2
        • Neurite growth promoting factor 2
        • Neurite outgrowth promoting protein
        • Neurite outgrowth-promoting factor 2
        • Neurite outgrowth-promoting protein
        • Retinoic acid inducible factor
        see all

      • Function

        Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.

      • Sequence similarities

        Belongs to the pleiotrophin family.

      • Cellular localization

        Secreted.
      • Information by UniProt

    Images

    • Western blot - Recombinant Human Midkine protein (ab49848)
      Western blot - Recombinant Human Midkine protein (ab49848)
      Anti-Midkine antibody (ab36038) at 1 µg/ml + Recombinant Human Midkine protein (ab49848) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 10 seconds

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab49848? Please let us know so that we can cite the reference in this datasheet.

    ab49848 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • Amphiregulin associated protein
      • Amphiregulin-associated protein
      • ARAP
      • FLJ27379
      • Mdk
      • Midgestation and kidney protein
      • Midkine
      • MK
      • MK 1
      • MK_HUMAN
      • MK1
      • NEGF 2
      • NEGF2
      • Neurite growth promoting factor 2
      • Neurite outgrowth promoting protein
      • Neurite outgrowth-promoting factor 2
      • Neurite outgrowth-promoting protein
      • Retinoic acid inducible factor
      see all

    • Function

      Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.

    • Sequence similarities

      Belongs to the pleiotrophin family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    • Western blot - Recombinant Human Midkine protein (ab49848)
      Western blot - Recombinant Human Midkine protein (ab49848)
      Anti-Midkine antibody (ab36038) at 1 µg/ml + Recombinant Human Midkine protein (ab49848) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 10 seconds

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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