Mouse Midkine ELISA Kit (ab279416)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.7 pg/ml
- Range: 37.5 pg/ml - 2400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Midkine ELISA Kit
See all Midkine kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 4.8% Inter-assay Sample n Mean SD CV% Plasma 3 5.86% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11.7 pg/ml -
Range
37.5 pg/ml - 2400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 82% - 110% Serum 115 108% - 120% Hep Plasma 105 94% - 114% EDTA Plasma 116 114% - 118% Cit plasma 99 94% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Midkine ELISA kit (ab279416) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Midkine protein in mouse serum, plasma and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Midkine with 11.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Midkine (MK), also known as neurite growth-promoting factor 2 (NEGF2), is a 13kDa heparin-binding growth factor or cytokine composed of two domains: The N-terminally located N-domain and the C-terminally located C-domain, which are connected by a hinge. The C-domain plays a role in neuronal development, whereas the N-domain is important for protein stability and dimerization. MK is involved in development, reproduction, repair, inflammation, innate immunity, control of blood pressure and angiogenesis. MK signaling is mediated by cell surface receptors as well as membrane proteins such as Protein Tyrosine Phosphatase ζ (PTPζ), low density lipoprotein receptor-related proteins (LRP), Notch2, integrins, anaplastic lymphoma kinase (ALK) and neuroglycan C. Due to its multifunctionality, MK has become an emerging target of drug development for the treatment of multiple diseases where studies show MK inhibitors may be useful in the treatment of cancer, multiple sclerosis, hypertension and osteoporosis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Midkine Capture Antibody 1 x 600µl 10X Mouse Midkine Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent CPR 1 x 6ml Mouse Midkine Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. -
Sequence similarities
Belongs to the pleiotrophin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Amphiregulin associated protein
- Amphiregulin-associated protein
- ARAP
see all -
Database links
- Entrez Gene: 17242 Mouse
- SwissProt: P12025 Mouse
- Unigene: 906 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of mouse Midkine standard curve in Sample Diluent NS + 1X Enhancer.The Midkine standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Midkine in mouse plasma and cell culture supernatant samples.The concentrations of Midkine were measured in duplicates, interpolated from the Midkine standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (heparin) 12.5% and kidney supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Midkine concentration was determined to be 3,044 pg/mL in plasma (heparin) and 6,948 pg/mL in and kidney supernatant.