Recombinant mouse IL-1 alpha protein (ab9725)
Key features and details
- Expression system: Escherichia coli
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant mouse IL-1 alpha protein
See all IL-1 alpha proteins and peptides -
Endotoxin level
Expression system
Escherichia coliProtein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Mouse -
Sequence
Recombinant Murine IL-1a is a 17.9 kDa protein containing 156 amino acid residues: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQ DVDKHY LSTTWLNDLQ QEVKFDMYAYSSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSKNYFTSAA YPE LFIATKEQSR VHLARGLPSM TDFQIS
Associated products
Specifications
Our Abpromise guarantee covers the use of ab9725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
The ED50, determined by the dose-dependent stimulation of murine D10S cells, is 5 x 108 units/mg. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
General Info
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Alternative names
- BAF
- FAF
- Hematopoietin 1
see all -
Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab9725 has been referenced in 1 publication.
- Dahllöf MS et al. The lysine deacetylase inhibitor Givinostat inhibits ß-cell IL-1ß induced IL-1ß transcription and processing. Islets 4:417-22 (0). PubMed: 23486342
Preparation and Storage
-
Alternative names
- BAF
- FAF
- Hematopoietin 1
see all -
Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
