Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)
![Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)](https://www.abcam.com/ps/products/223/ab223584/Images/ab223584-445530-anti-cbl-cblb-antibody-ye315-bsa-and-azide-free-western-blot.jpg "Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [YE315] to CBL + CBLB - BSA and Azide free
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CBL + CBLB antibody [YE315] - BSA and Azide free -
Description
Rabbit monoclonal [YE315] to CBL + CBLB - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen sequence is 100% identical for both CBL and CBLB, therefore there is likely to be cross-reactivity.
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Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HuT-78, HeLa and Jurkat cell lysate. IHC-P: Human uterus adenocarcinoma and colon tissues. ICC/IF: Jurkat cells.
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General notes
ab223584 is the carrier-free version of ab32425 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab223584 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YE315 -
Isotype
IgG -
Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Images
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Western blot - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)Anti-CBL + CBLB antibody [YE315] (ab32425) at 1/20000 dilution (purified) + HeLa cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab32425, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)](https://www.abcam.com/ps/products/223/ab223584/Images/ab223584-5-anti-cbl-cblb-antibody-ye315-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)This data was developed using ab32425, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling CBL + CBLB with unpurified ab32425 at a dilution of 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)](https://www.abcam.com/ps/products/223/ab223584/Images/ab223584-4-anti-cbl-cblb-antibody-ye315-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)This data was developed using ab32425, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling CBL + CBLB with purified ab32425 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)](https://www.abcam.com/ps/products/223/ab223584/Images/ab223584-3-anti-cbl-cblb-antibody-ye315-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)This data was developed using ab32425, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling CBL + CBLB with purified ab32425 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. -
![Western blot - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)](https://www.abcam.com/ps/products/223/ab223584/Images/ab223584-403187-anti-cbl-cblb-antibody-ye315-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)Anti-CBL + CBLB antibody [YE315] (ab32425) at 1/5000 dilution (purified) + HuT-78 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab32425, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)](https://www.abcam.com/ps/products/223/ab223584/Images/ab223584-397869-anti-cbl-cblb-antibody-ye315-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)Anti-CBL + CBLB antibody [YE315] (ab32425) at 1/500 dilution (unpurified) + Jurkat cell lysate
Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab32425, the same antibody clone in a different buffer formulation.
-
![Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)](https://www.abcam.com/ps/products/223/ab223584/Images/ab223584-6-benefits-of-recombinant-antibodies.png)
Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)