Recombinant human Yes1 protein (ab196076)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies
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Product name
Recombinant human Yes1 protein
See all Yes1 proteins and peptides -
Biological activity
Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated.
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Purity
>= 70 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTA VNFSSLSMTPFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYD YEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPAD SIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDW DEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKL TTVCPTVKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTK VAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFM SKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAAN ILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTI KSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESL HELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPGENL -
Predicted molecular weight
91 kDa including tags -
Molecular weight information
88 kDa by SDS-PAGE -
Amino acids
2 to 543 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_005433.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Active human Yes1 full length protein (ab196076)
Specific activity of ab196076 was determined to be 200 pmol/min/µg in a kinase assay using Poly-(Glu4:Tyr)-biotin as substrate.
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SDS-PAGE - Active human Yes1 full length protein (ab196076)SDS-PAGE analysis of ab196076.