PP2, Src and RIP2 kinase inhibitor (ab120308)
Key features and details
- Src and RIP2 kinase inhibitor
- CAS Number: 172889-27-9
- Purity: > 98%
- Soluble in DMSO to 50 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
PP2, Src and RIP2 kinase inhibitor -
Description
Src and RIP2 kinase inhibitor -
Purity
> 98% -
CAS Number
172889-27-9 -
Chemical structure
Properties
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Chemical name
1-tert-Butyl-3-(4-chlorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine -
Molecular weight
301.78 -
Molecular formula
C15H16ClN5 -
PubChem identifier
4878 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 50 mM and in ethanol to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Clc1ccc(cc1)c3nn(c2ncnc(N)c23)C(C)(C)C -
Source
Synthetic
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Research areas
Images
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Functional Studies - PP2, Src and RIP2 kinase inhibitor (ab120308)ab11512 staining E-Cadherin in SW480 cells treated with PP2 (ab120308), by ICC/IF. Increase in E-cadherin expression correlates with increased concentration of PP2, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120308 (PP2) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab96971) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
