Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [LynY507-5B6] to Lyn (phospho Y507)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-Lyn (phospho Y507) antibody [LynY507-5B6]
See all Lyn primary antibodies
Description

Rabbit monoclonal [LynY507-5B6] to Lyn (phospho Y507)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Lyn (phospho Y507). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P07948

Run BLAST with Run BLAST with
Positive control
- WB: HeLa (treated with hydrogen peroxide) cell extract. Flow cyt: C2C12 cells (treated with pervanadate); Jurkat cells (treated with IFNa + IL-4 + pervanadate).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

LynY507-5B6
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using ab278639 at 0.01µg/mL.
Western blot - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

All lanes : Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

Lane 1 : HeLa cells treated with H2O2. Cell lysates were ran on a SDS-PAGE gel, transferred to nitrocellulose membrane, blocked, non-treated (-) and stained.
Lane 2 : HeLa cells treated with H2O2. Cell lysates were ran on a SDS-PAGE gel, transferred to nitrocellulose membrane, blocked and treated with lambda phosphatase (+) and stained.

Predicted band size: 59 kDa
Western blot - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

All lanes : Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639) at 0.01 µg/ml

Lane 1 : HeLa cell extract, treated with H2O2
Lane 2 : HeLa cell extract, untreated

Predicted band size: 59 kDa
Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278639 at 0.01µg/mL.
Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278639 at 0.01µg/mL.
Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

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