Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [LynY507-5B6] to Lyn (phospho Y507)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Lyn (phospho Y507) antibody [LynY507-5B6]
    See all Lyn primary antibodies

  • Description

    Rabbit monoclonal [LynY507-5B6] to Lyn (phospho Y507)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Lyn (phospho Y507). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P07948

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa (treated with hydrogen peroxide) cell extract. Flow cyt: C2C12 cells (treated with pervanadate); Jurkat cells (treated with IFNa + IL-4 + pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    LynY507-5B6

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using ab278639 at 0.01µg/mL.
  • Western blot - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Western blot - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    All lanes : Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

    Lane 1 : HeLa cells treated with H2O2. Cell lysates were ran on a SDS-PAGE gel, transferred to nitrocellulose membrane, blocked, non-treated (-) and stained.
    Lane 2 : HeLa cells treated with H2O2. Cell lysates were ran on a SDS-PAGE gel, transferred to nitrocellulose membrane, blocked and treated with lambda phosphatase (+) and stained.

    Predicted band size: 59 kDa

  • Western blot - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Western blot - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    All lanes : Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639) at 0.01 µg/ml

    Lane 1 : HeLa cell extract, treated with H2O2
    Lane 2 : HeLa cell extract, untreated

    Predicted band size: 59 kDa

  • Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

    Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278639 at 0.01µg/mL. 

  • Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Flow Cytometry - Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278639 at 0.01µg/mL.
  • Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)
    Anti-Lyn (phospho Y507) antibody [LynY507-5B6] (ab278639)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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