Recombinant Human TIM 3 protein (Fc Chimera) (ab224631)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 85% SDS-PAGE
- Endotoxin level:
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 0.75% Glycine, 5% Trehalose, 0.61% Tris, Sodium chloride, L-Arginine
Lyophilized from 0.22 µm filtered solution. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE of reduced ab224631 stained overnight with Coomassie Blue. The protein migrates as 60-80 kDa due to glycosylation.
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Immobilized ab224631 at 2 μg/ml (100 µl/well), can bind anti-TIM3 mAb with a linear range of 0.2 - 2 ng/mL.