Recombinant human TIM 3 protein (Active) (ab182714)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, ELISA
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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ELISA - Recombinant Human TIM 3 protein (ab182714)
Immobilized ab182714 at 0.2 μg/mL (100 μL/well) can bind Anti-TIM3 MAb, Human IgG4 with a linear range of 0.2-6 ng/mL.
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Functional Studies - Recombinant Human TIM 3 protein (ab182714)Anti-Human TIM-3 MAb (human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind ab182714 with an affinity constant of 9.7 nM as determined in a SPR assay.
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SDS-PAGE - Recombinant Human TIM 3 protein (ab182714)DTT-reduced (+) SDS-PAGE analysis of ab182714, stained overnight with Coomassie Blue. The protein migrates as 45-50 kDa due to glycosylation.
