Recombinant human RAGE protein (Fc Chimera Active) (ab219875)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 60% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.75% Glycine, 5% Trehalose, 0.61% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE - Recombinant human RAGE protein (Fc Chimera Active) (ab219875)
ab219875 on SDS-PAGE under reducing (R) conditions. The gel was stained overnight with Coomassie Blue. The protein migrates as 80-90 kDa and 35 kDa Fc fragment under reducing (R) conditions due to glycosylation and auto-cleavage.
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Functional Studies - Recombinant human RAGE protein (Fc Chimera Active) (ab219875)Immobilized ab219875 at 20 μg/mL (100 μL/well) can bind Human HMGB1, His Tag with a linear range of 0.313-5 μg/mL.
