Canine RAGE ELISA Kit (ab273182)
Key features and details
- Sensitivity: 16 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine RAGE ELISA Kit
See all RAGE kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 10% Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
16.38 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93.64 85% - 101% Plasma 80.58 68% - 95% Cell culture media 102.4 93% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine RAGE ELISA Kit (ab273182) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine RAGE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Canine RAGE coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine RAGE Antibody 2 vials Canine RAGE Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- Advanced glycosylation end product-specific receptor
- Ager
- DAMA 358M23.4
see all -
Database links
Images
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Canine RAGE ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.