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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid

Human SAP Antibody Pair - BSA and Azide free (ab256682)

Human SAP Antibody Pair - BSA and Azide free (ab256682)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human SAP Antibody Pair - BSA and Azide free
    See all Serum Amyloid P/SAP kits
  • Assay type

    ELISA set
  • Range

    468.75 pg/ml - 30000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human SAP. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab246537), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human SAP Capture Antibody (unconjugated) 1 x 100µg
    Human SAP Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Cardiovascular
    • Blood
    • Serum Proteins
  • Function

    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificity

    Found in serum and urine.
  • Involvement in disease

    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similarities

    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications

    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02743 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 9.5S alpha 1 glycoprotein
    • 9.5S alpha-1-glycoprotein
    • Amyloid P component serum
    • APCS
    • MGC88159
    • Pentaxin related
    • Pentraxin 2
    • PTX 2
    • PTX2
    • SAMP_HUMAN
    • SAP
    • Serum Amyloid P
    • Serum amyloid P component
    • Serum amyloid P-component(1-203)
    see all
  • Database links

    • Entrez Gene: 325 Human
    • Omim: 104770 Human
    • SwissProt: P02743 Human
    • Unigene: 507080 Human

    Images

    • Sandwich ELISA - Human SAP Antibody Pair - BSA and Azide free (ab256682)
      Sandwich ELISA - Human SAP Antibody Pair - BSA and Azide free (ab256682)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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      Applications:

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